CD34
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| Location (UCSC) | Chr 1: 207.88 – 207.91 Mb | Chr 1: 194.62 – 194.64 Mb | |||||||
| PubMed search | [3] | [4] | |||||||
| View/Edit Human | View/Edit Mouse |
CD34 is a transmembrane phosphoglycoprotein protein encoded by the CD34 gene in humans, mice, rats and other species.[5][6][7]
CD34 derives its name from the
Function
The CD34 protein is a member of a family of single-pass transmembrane
CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. It is expressed on lymph node endothelia, whereas the L-selectin to which it binds is on the T cell.[15][16] Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina.[17][18] Finally, recent data suggest CD34 may also play a more selective role in chemokine-dependent migration of eosinophils and dendritic cell precursors.[19][20] Regardless of its mode of action, under all circumstances CD34, and its relatives podocalyxin and endoglycan, facilitates cell migration.[13][19]
Tissue distribution
Cells expressing CD34 (CD34+ cell) are normally found in the
It is important to mention that Long-Term Haematopoietic Stem Cells (LT-HSCs) in mice and humans are the haematopoietic cells with the greatest self-renewal capacity and were shown to be CD34+ and CD38− cell fraction within the lineage-depleted cell population (LIn−).[21][22] Human HSCs express the CD34 marker.[21][23] Later studies have reported that low rhodamine retention identifies LT-HSCs within the Lin−CD34+CD38− population.[24][25][26]
CD34 is expressed in roughly 20% of murine haematopoietic stem cells,[27] and can be stimulated and reversed.[28]
Clinical applications
CD34+ is often used clinically to quantify the number of haemopoietic stem cells for use in haemopoietic stem cell transplantation. This is generally a useful marker for cell dosing although there is some evidence that the CD34+ quantification may not be reliable in some circumstances.[29] CD34+ cells may be isolated from blood samples using immunomagnetic techniques and used for CD34+ transplants, which have lower rates of graft-versus-host disease.[30]
Antibodies are used to quantify and purify
Cells observed as CD34+ and CD38- are of an
In tumors, CD34 is found in
A negative CD34 may exclude
Injection of CD34+
Interactions
CD34 has been shown to
See also
References
- ^ a b c GRCh38: Ensembl release 89: ENSG00000174059 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000016494 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Entrez Gene: CD34 CD34 molecule".
- PMID 1370171.
- PMID 1374051.
- PMID 6586833.
- PMID 3857402.
- ^ Tindle RW. Katz F. Martin H. Watt D. Catovsky D. Janossy G. Greaves M. (1987). "BI-3C5 (CD34) defines multipotential and lineage restricted progenitor cells and their leukaemic counterparts ". In 'Leucocyte Typing 111: White Cell Differentiation Antigens. Oxford University Press, 654-655.
- ^ Loken M. Shah V. Civin CI.. (1987). "Characterization of myeloid antigens on human bone marrow using multicolour immunofluorescence". In: McMichael, Leucocyte Typing III:White Cell Differentiation Antigens.Oxford University Press 630-635.
- ^ PMID 24497003.
- ^ S2CID 18154561.
- S2CID 41420678.
- PMID 9466573.
- S2CID 7630223.
- PMID 15664158.
- PMID 19853564.
- ^ S2CID 10999494.
- PMID 21642249.
- ^ PMID 10590054.
- PMID 11782553.
- S2CID 10040999.
- S2CID 25804835.
- PMID 8513861.
- PMID 14563317.
- S2CID 83284239.
- PMID 10961905.
- PMID 31697810.
- PMID 29325829.
- ^ Srivastava A, Bapat M, Ranade S, Srinivasan V, Murugan P, Manjunath S, Thamaraikannan P, Abraham S (2010). "Autologous Multiple Injections of in Vitro Expanded Autologous Bone Marrow Stem Cells For Cervical Level Spinal Cord Injury - A Case Report". Journal of Stem Cells and Regenerative Medicine.
- S2CID 5649484.
- S2CID 27251276.
- PMID 11389015.
Further reading
- Bellini A, Mattoli S (September 2007). "The role of the fibrocyte, a bone marrow-derived mesenchymal progenitor, in reactive and reparative fibroses". Laboratory Investigation; A Journal of Technical Methods and Pathology. 87 (9): 858–870. PMID 17607298.
- Simmons DL, Satterthwaite AB, Tenen DG, Seed B (January 1992). "Molecular cloning of a cDNA encoding CD34, a sialomucin of human hematopoietic stem cells". Journal of Immunology. 148 (1): 267–271. PMID 1370171.
- Satterthwaite AB, Burn TC, Le Beau MM, Tenen DG (April 1992). "Structure of the gene encoding CD34, a human hematopoietic stem cell antigen". Genomics. 12 (4): 788–794. PMID 1374051.
- Fina L, Molgaard HV, Robertson D, Bradley NJ, Monaghan P, Delia D, et al. (June 1990). "Expression of the CD34 gene in vascular endothelial cells". Blood. 75 (12): 2417–2426. PMID 1693532.
- Fackler MJ, Civin CI, Sutherland DR, Baker MA, May WS (July 1990). "Activated protein kinase C directly phosphorylates the CD34 antigen on hematopoietic cells". The Journal of Biological Chemistry. 265 (19): 11056–11061. PMID 1694174.
- Sutherland DR, Watt SM, Dowden G, Karhi K, Baker MA, Greaves MF, Smart JE (December 1988). "Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34". Leukemia. 2 (12): 793–803. PMID 2462139.
- Nakamura Y, Komano H, Nakauchi H (February 1993). "Two alternative forms of cDNA encoding CD34". Experimental Hematology. 21 (2): 236–242. .
- Huyhn A, Dommergues M, Izac B, Croisille L, Katz A, Vainchenker W, Coulombel L (December 1995). "Characterization of hematopoietic progenitors from human yolk sacs and embryos". Blood. 86 (12): 4474–4485. PMID 8541536.
- Tavian M, Coulombel L, Luton D, Clemente HS, Dieterlen-Lièvre F, Péault B (January 1996). "Aorta-associated CD34+ hematopoietic cells in the early human embryo". Blood. 87 (1): 67–72. PMID 8547678.
- Hillier LD, Lennon G, Becker M, Bonaldo MF, Chiapelli B, Chissoe S, et al. (September 1996). "Generation and analysis of 280,000 human expressed sequence tags". Genome Research. 6 (9): 807–828. PMID 8889549.
- Uchida N, Yang Z, Combs J, Pourquié O, Nguyen M, Ramanathan R, et al. (April 1997). "The characterization, molecular cloning, and expression of a novel hematopoietic cell antigen from CD34+ human bone marrow cells". Blood. 89 (8): 2706–2716. PMID 9108388.
- Ruiz ME, Cicala C, Arthos J, Kinter A, Catanzaro AT, Adelsberger J, et al. (October 1998). "Peripheral blood-derived CD34+ progenitor cells: CXC chemokine receptor 4 and CC chemokine receptor 5 expression and infection by HIV". Journal of Immunology. 161 (8): 4169–4176. PMID 9780190.
- Kees UR, Ford J (February 1999). "Synergistic action of stem-cell factor and interleukin-7 in a human immature T-cell line". Immunology. 96 (2): 202–206. PMID 10233696.
- Bistrup A, Bhakta S, Lee JK, Belov YY, Gunn MD, Zuo FR, et al. (May 1999). "Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligands for L-selectin". The Journal of Cell Biology. 145 (4): 899–910. PMID 10330415.
- Lataillade JJ, Clay D, Dupuy C, Rigal S, Jasmin C, Bourin P, Le Bousse-Kerdilès MC (February 2000). "Chemokine SDF-1 enhances circulating CD34(+) cell proliferation in synergy with cytokines: possible role in progenitor survival". Blood. 95 (3): 756–768. PMID 10648383.[permanent dead link]
- Felschow DM, McVeigh ML, Hoehn GT, Civin CI, Fackler MJ (June 2001). "The adapter protein CrkL associates with CD34". Blood. 97 (12): 3768–3775. PMID 11389015.
- Dobo I, Robillard N, Pineau D, Geneviève F, Piard N, Rapp MJ, et al. (November 2001). "Use of pathology-specific peripheral blood CD34 thresholds to predict leukapheresis CD34 content with optimal accuracy: a bicentric analysis of 299 leukaphereses". Annals of Hematology. 80 (11): 639–646. S2CID 23018429.
- Hogan CJ, Shpall EJ, Keller G (January 2002). "Differential long-term and multilineage engraftment potential from subfractions of human CD34+ cord blood cells transplanted into NOD/SCID mice". Proceedings of the National Academy of Sciences of the United States of America. 99 (1): 413–418. .
- Krauter J, Hartl M, Hambach L, Kohlenberg A, Gunsilius E, Ganser A, Heil G (December 2001). "Receptor-mediated endocytosis of CD34 on hematopoietic cells after stimulation with the monoclonal antibody anti-HPCA-1". Journal of Hematotherapy & Stem Cell Research. 10 (6): 863–871. PMID 11798512.
- Okuno Y, Iwasaki H, Huettner CS, Radomska HS, Gonzalez DA, Tenen DG, Akashi K (April 2002). "Differential regulation of the human and murine CD34 genes in hematopoietic stem cells". Proceedings of the National Academy of Sciences of the United States of America. 99 (9): 6246–6251. PMID 11983914.
- Hotfilder M, Röttgers S, Rosemann A, Jürgens H, Harbott J, Vormoor J (July 2002). "Immature CD34+CD19- progenitor/stem cells in TEL/AML1-positive acute lymphoblastic leukemia are genetically and functionally normal". Blood. 100 (2): 640–646. PMID 12091359.
External links
- Antigens,+CD34 at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
- Mouse CD Antigen Chart
- Human CD Antigen Chart
- Human CD34 genome location and CD34 gene details page in the UCSC Genome Browser.