Neurogenins

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Neurogenins, often abbreviated as Ngn, are a family of

transcription factors involved in specifying neuronal differentiation. The family consisting of Neurogenin-1, Neurogenin-2, and Neurogenin-3, plays a fundamental role in specifying neural precursor cells and regulating the differentiation of neurons during embryonic development. It is one of many gene families related to the atonal gene in Drosophila. Other positive regulators of neuronal differentiation also expressed during early neural development include NeuroD and ASCL1.[1]

Function

Neurogenins primarily govern the transition of neural progenitor cells to neurons by activating specific downstream genes associated with neuronal differentiation. Their involvement spans various stages of neurogenesis, including the determination of neural progenitor identity, cell cycle exit, and the acquisition of neuronal characteristics. Notably, Neurogenins influence the specification of different neuronal subtypes, contributing to the diverse array of neurons within the central and peripheral nervous systems.[2]

In

dorsal root ganglia and development of the sensory lineage.[3][4]

Regulation of neurogenic cascades

The activity of Neurogenins is intricately regulated by molecular pathways and environmental cues. Interaction with other transcription factors, such as proneural factors and Notch signaling, further refines the neurogenic cascades. Their spatiotemporal expression patterns and cross-regulation contribute to the exquisite precision required for proper neural development.[5][6]

Clinical implications

Neurogenins holds significant implications for clinical research, particularly in the context of

neurodevelopmental disorders and neurological regeneration.[7] Aberrations in the expression or regulation of Neurogenins have been linked to conditions such as autism spectrum disorders and neurodegenerative diseases.[8]
Ongoing research continues to explore the therapeutic potential of manipulating Neurogenin activity for neural repair and regeneration.

Neurogenin-1

Main article: Neurogenin-1

Neurogenin 1 (Ngn1) is a Class-A

dimerize with another bHLH protein.[9] Ngn1 is a proneural gene because its expression is seen prior to neural lineage determination, indicating it plays a role in neuronal differentiation.[1]

Neuronal differentiation

In E14 rats, when Ngn1 is present in the

Smad1 transcriptional co-activator complex, which recruits it to the enhancer box upstream of the gene in the promoter for neuronal genes. Binding of Ngn1, to the enhancer box, induces the transcription factor NeuroD to bind to its own enhancer boxes, inducing the genes involved in neuronal differentiation.[10]

Regulation by BMP

homeodomain protein expression. This is critical for the initiation of neurogenesis.[11]

Regulation by LIF

In the presence of Ngn1, the

transcription through binding the STAT1/3 complex, which is activated through the LIF pathway.[10]

Glial differentiation

Along with supporting neuronal differentiation, when expressed in embryonic neural tissue, Ngn1 also acts to inhibit glial differentiation.[12] In the absence of Ngn1, the CBP/p300/Smad1 transcriptional co-activator complex is recruited to and binds to activated STAT1/3, which in turn causes the expression of GFAP, causing glial differentiation. In the presence of Ngn1, inhibition of gliogenesis occurs through Ngn1 binding to the CBP/p300/Smad1 transcriptional co-activator complex, recruiting it away from STAT1/3.[10]

Regulation by BMP

In cases of low levels of Ngn1, BMPs promote glial differentiation. Since Ngn1 is the limiting factor, CBP/p300/Smad1 is able to interact with STAT1/3 and induce gliogenesis.[10]

Regulation by Notch

Activation of the notch pathway, causes the inhibition of proneural bHLH genes, such as Ngn1, which allows for the CBP/p300/Smad1 to interact with STAT1/3 and induce gliogenesis.[10] Along with the embryonic rat, it was also seen in zebrafish that the repression of Ngn1 by Notch, promotes glial lineage in neural crest and central nervous system formation through the inhibition of neuronal differentiation.[1][13] In addition to the Notch pathway activating the transcriptional factors involved in the promotion of gliogenesis, it is possible that these same factors are involved in the inhibition of other fates.

Regulation by LIF

In the absence of Ngn1, the LIF pathway is able to activate STAT1/3, which allows for the promotion of GFAP transcription via the STAT binding site. The promotion of GFAP transcription induced glial differentiation.[10]

Neurogenin-2

Main article: Neurogenin-2

Neurogenin 2 (Ngn2) is a bHLH transcription factor involved in both neurogenesis and neural specification. This protein binds to enhancer-box regulatory elements on the promoters of many genes related to neurogenesis and neural specification. For sufficient DNA binding, Ngn2 must form a

dimer with an enhancer protein.[14]

Neurogenesis and glial inhibition

Ngn2 is a transcription factor that both increases expression of proneural genes and drives neural fate by inhibiting expression of glial genes in

Nkx2.2, which inhibits proneural genes, is upregulated.[15] Glial fate switch was reduced by inhibiting Nkx2.2 and Olig2 in neural progenitors while allowing the expression of Ngn2. The ability of Olig2 to induce expression of Ngn2 is reduced when Nkx2.2 is expressed.[16]

Neural specification

Mice lacking Ngn2 have fewer

interneurons, indicating that Ngn2 plays a role in specification of these neurons.[17]

Pan-neuronal fate

Heterodimerized Ngn2/enhancer protein complex can bind to enhancer boxes to promote transcription of genes related to a non-specified neuronal fate.[17]

V2 interneuron fate

When an enhancer box of a promoter that has been bound by the Ngn-2/enhancer protein complex is also bound by a dimer of the adaptor nuclear LIM interactor (NLI) bound to two LIM homeobox protein 3 (Lhx3), genes related to V2 interneuron identity are expressed.[17]

Motor neuron fate

A dimer of the adaptor NLI bound to two islet 1 (Isl1) proteins and each Isl1 is bound by Lhx3 is called the LIM-homeodomain (LIM-HD) transcription complex. When an enhancer box of a promoter that has been bound by the heterodimerized Ngn2/E-protein complex, the LIM-HD transcription complex is able to bind to drive expression of genes related to motor neuron fate, but only if Ngn2 has been properly phosphorylated.[17]

Ngn2 has two

glycogen synthase kinase 3 (Gsk3). Phosphorylation of Ngn2 enables interaction with LIM-homeodomain proteins, leading to ventral neural fate and motor neuron specification.[18] The importance of this phosphorylation was determined by using mice that express a mutated form of Ngn2 protein which has the serines from the previously mentioned phosphorylation sites mutated into alanines, which cannot be phosphorylated. These mutant mice have a decreased number of motor neurons and an increased number of V2 interneurons, suggesting that phosphorylation is necessary for driving expression of genes related to motor neuron fate but not V2 interneuron fate and non-specified neural fate.[17]

Neurogenin-3

Main article: Neurogenin-3

Neurogenin 3 (Ngn3) is another member of the bHLH family of transcription factors. Ngn3 functions in the differentiation of

endocrine pancreas cells. Although its key function is in the pancreas, intestinal cells and neural cells express Ngn3 as well. Several studies have highlighted the importance of Ngn3 for differentiation of endocrine cells. In mice, Ngn3 is present in cells as the pancreas begins to bud and glucagon cells are formed. There are several pathways that Ngn3 works through.[19][20][21][22]

Ngn3 is a crucial component in pancreatic development and plays a supporting role in intestinal as well as neuronal cell development. Studies have demonstrated that knockout of Ngn3 in mice leads to death shortly after birth possibly due to after effects of severe diabetes.[19] Further studies are taking place to investigate Ngn3's possible role as a treatment for diabetes and regeneration of cells in the pancreas.[19][21]

Neurogenin 3 (NGN3) is expressed by 2-10% of acinar and duct cells in the histologically normal adult human pancreas. NGN3+ cells isolated from cultured exocrine tissue by coexpressed cell surface glycoprotein CD133 have a transcriptome consistent with exocrine dedifferentiation, a phenotype that resembles endocrine progenitor cells during development, and a capacity for endocrine differentiation in vitro.[23] Human [24] and rodent [25][26][27][28][29][30][31][32][33] exocrine cells have been reprogrammed into cells with an islet cell-like phenotype following direct expression of NGN3 or manipulation that leads to its expression.

Phases of pancreatic development

The development of the pancreas is broken up into three phases, primary phase, secondary phase, and tertiary phase. Ngn3 is active in the primary and secondary phase. In the primary phase Ngn3 assists in

pancreatic progenitor cells to become an endocrine multipotent pro-precursor.[19]

Modulation via notch pathway

The Notch pathway is one of the key modulators of Ngn3. The binding of

Delta and Serrate, activation ligands for the Notch pathway, activates the Notch surface molecule. This allows the Notch intracellular domain to activate RBK-Jκ to translocate into the nucleus. This complex then activates hairy and enhancer of split (HES)-type proteins, which are inhibitors of Ngn3. The cells that allow the Notch/RBK-Jκ complex to enter are the ones that will not be differentiated into pancreatic cells because Ngn3 is suppressed. It is important to mention that Ngn3 has three HES1 binding sites adjacent to the TATA box sequence that allow for the regulation of this transcription factor.[19]

Downstream targets of Ngn3

NeuroD

Ngn3 can also activate the neurogenic differentiation factor 1(NeuroD1) like most of its other family members through the enhancer boxes present in its structure. Being that NeuroD1 is expressed along with Ngn3 in differentiating cells, it is considered one of the transcription factors downstream targets.[19]

Pax4

Another important target is

paired box gene 4 (Pax4), which plays a major role in β cell and δ cell differentiation. Ngn3 works hand-in-hand with HNF1α to activate the Pax4 promoter to induce specific cell differentiation.[19]

Nkx2.2

Another transcription factor that may be a downstream target of Ngn3 is Nkx2.2 because it is often coexpressed with it. Studies have shown that disrupting Nkx2.2 expression results in problems with α and β cell differentiation.[20][21]

References

  1. ^
    PMID 9570134
    .
  2. PMID 34971564
    .
  3. ISBN 0-306-48330-0
    .
  4. PMID 10398684
    .
  5. PMID 21412988
    .
  6. PMID 32661334
    .
  7. PMID 19789989
    .
  8. PMID 32273286
    .
  9. ^ Universal protein resource accession number Q92886 for "Neurogenin-1" at UniProt.
  10. ^
    S2CID 15885798
    .
  11. S2CID 573477
    .
  12. ^ "Neurogenin-1 Products: R&D Systems". www.rndsystems.com. Archived from the original on 2014-01-16.
  13. S2CID 10863124
    .
  14. ^ Universal protein resource accession number Q9H2A3 at UniProt.
  15. ISBN 978-0-12-374539-2
    .
  16. S2CID 2624758
    .
  17. ^
    S2CID 13530075
    .
  18. PMID 18400164
    .
  19. ^
    PMID 21099270
    .
  20. ^
    PMID 21824251
    .
  21. ^
    PMID 15256770
    .
  22. S2CID 691004
    .
  23. PMID 26288179
    .
  24. PMID 22606327
    .
  25. S2CID 8058714
    .
  26. PMID 23470530
    .
  27. PMID 22828498
    .
  28. PMID 20663919
    .
  29. PMID 24240391
    .
  30. PMID 16514419
    .
  31. PMID 25476775
    .
  32. S2CID 205214877
    .
  33. PMID 25105579
    .
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